Researchers Identify Mechanism that Helps Bacteria Avoid Destruction in Cells
Infectious
diseases currently cause about one-third of all human deaths worldwide,
more than all forms of cancer combined. Advances in cell biology and
microbial genetics have greatly enhanced understanding of the cause and
mechanisms of infectious diseases. Researchers from Thomas Jefferson University, the Pasteur Institute in Paris, and Yale University reported in PLoS ONE, a way in which intracellular pathogens exploit the biological attributes of their hosts in order to escape destruction.
Intracellular pathogens include Chlamydia, which causes infertility in women,and Legionella,
which causes Legionnaire’s disease. These pathogens are able to escape
destruction and remain in the cells. Until now, it was unclear how they
were able avoid the destruction process. The team of researchers, led
by Fabienne Paumet, Ph.D., assistant professor of Microbiology and
Immunology at Jefferson Medical College of Thomas Jefferson University,
found that it appears to be due to SNARE-like proteins expressed by the
pathogen.
SNARE
proteins are necessary for eukaryotic cells to fuse to their
intracellular compartments. These proteins, which are present on the
surface of almost all intracellular compartments, interact to form a
stable complex, triggering fusion of the membranes. Intracellular
pathogens, like Chlamydia and Legionella, must contend with vesicular trafficking and membrane fusion in the host cell. But they manage to bypass the lysosome, where
other pathogens would normally be destroyed.
The researchers tested the hypothesis that SNARE-like proteins expressed by the bacteria themselves were capable to interact
with the eukaryotic SNAREs and alter membrane fusion to their advantage. The Chlamydia bacteria expressed a SNARE-like protein called IncA and the Legionella expressed a SNARE-like protein called IcmG/DotF, both of which inhibit SNARE-protein-mediated fusion.
“Based
on our results, it seems that intracellular bacteria are able to
express ‘inhibitory SNAREs’ to block fusion between the lysosome and
the compartment containing the bacteria,” Dr. Paumet said. “The SNARE
proteins function like a zipper, and without each half, they can’t
fuse.”
SNARE-like bacterial proteins would appear to be a viable therapeutic target, since disruption of their protective function
should render intracellular bacteria more susceptible to clearance from the phagosome.
“Thorough
understanding of the bacterial SNARE-like protein system will give us
the necessary tools to design such therapeutics,” Dr. Paumet said.
Media Only Contact:
Emily Shafer
Thomas Jefferson University Hospital
Phone: (215) 955-6300
Published: 10/9/2009